Microplastics and microfibres are found ubiquitously in global oceans as well as marine organisms from different trophic levels. Howeverlittle is known about the presence of microplastics and microfibres in marine megafaunasuch as sharks. This study provided the first investigation of the presence of microplastics and other anthropogenic fibres (i.e.cellulose based fibres) in intestine and muscle samples of four large apex shark species in Australian coastal waters. Microplastics and other anthropogenic fibres were found in 82% of the analysed intestine samples. The mean abundance in intestine samples was 3.1 ± 2.6 particles/individualwhich corresponded to 0.03 ± 0.02 particles/g of intestineacross all shark species. The size of particles ranged from 190 to 4860 μm in length with 92% being fibrous in shape and the rest fragments. FTIR spectroscopy identified that 70% of fibres were cellulose-based followed by polyethylene terephthalate (PET)while the fragments were polyethylene and polypropylene. In shark muscles60% of samples contained microplastics and other anthropogenic fibresagain with the majority being cellulose-based fibres followed by PET fibres. Methodological differences hinder a more comprehensive assessment of microplastic contamination across studies. Additionallywe identified some challenges which should be factored in for future studies looking at the presence of microplastics as well as other anthropogenic fibres in these large marine organisms. Overallthe findings provide first evidence of microplastics and other anthropogenic fibres not only in the intestinesbut also in muscle tissues of large apex shark species.