Flow Induced Dispersion Analysis

Fida Evolve: Faster insights – faster decisions

The Fida's unique capability for measuring binding Kd from weak mM level to strong pM level really enhances our bioanalytical capabilities.

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The Fida's unique capability for measuring binding Kd from weak mM level to strong pM level really enhances our bioanalytical capabilities.

What we value about FIDA is the amount of information we can get from a tiny amount of protein in a single QC run. FIDA technology enables us to understand our proteins better - particularly when they behave in unexpected ways.

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I am convinced. I have never before been able to detect the ternary construct formation of my construct directly in cell lysate – it took less than two hours.

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I am convinced. I have never before been able to detect the ternary construct formation of my construct directly in cell lysate – it took less than two hours.

Compress weeks of biophysics into a few routine runs.

Fida Evolve resolves molecular sizebindingand sample characteristics from one in-solution diffusion profile - replacing a stack of complementary assays with a single experiment. Multiple questionsone assay. Data gaps closed in the same run that raises them. Routines that used to take daysfinished in minutes.

Robustness and confidence of

3 simultaneous measurements

High data yield

10 biophysical readouts

Early binder screening and ranking with

1-minute Kd

Multiple readouts per run

Fewer runs

Faster time to insights

Faster decisions

Higher ROI

Fida Evolve:

Our new multiparameter FIDA biophysics platform enables you to collapse weeks of biophysical analyses into a few days work.

Learn about the new Fida Evolve

Developed for Complex Samples and New Modalities:

For FIDA the sample environment is contextnot obstacle. It allows you to increase biological relevance by handling unpurified sampleshigh-background matricesand heterogeneous populationsdistinguishing bound/unbound species and oligomeric states without separation steps.

Delivers binding data in context of sample integrity:

By reporting binding alongside aggregationsizeand quality metrics from the same assayFIDA eliminates ambiguity caused by fragmented assays.

Wide Affinity & Kinetic Range:

Measures dissociation constants from picomolar (pM) to millimolar (mM)and kinetic processes from seconds to hourscovering weak bindersfragmentsor biologics alike.

Sample and time efficient:

nL–µL sample sizesno need for purificationmultiple simultaneous readouts & calibration-free operation - all this combined with AI-compatible data reporting allows you to efficiently move frontiers.

FIDA in numbers

Over

Characterization parameters

Affinity of

pM - mM

Strong and weak binders

40 nL Analyte

Down to nL sample volumes

Kinetics

Sec-hrs

Fast and slow

Size accuracy of

Size change

2 x 96 samples

Fully Automated

Between

100mM - 10pM

Binding affinities

3 Wavelengths available:

480nm640nm or 280nm LED

Size detection of

0.5 - 500nm

Size Range

Size-based quality control: a wealth of information to accelerate your research

Size measurements are the fundament for over 10 parameters delivered by FIDA. Detection of hydrodynamic radiuschanges in hydrodynamic radius and apparent hydrodynamic radius provide the measurements used not only for the Sizebut also for Binding & AffinityBinding KineticsQuantityand Quality parameterswhich you can read more about:

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